Flavor-Food Compound Interactions by NMR Spectroscopy

Abstract

A review. The most popular method of NMR used to investigate interactions of a specific ligand-macromol. complex is based on the chem. shift changes (and/or on resonance broadening) of the macromol. using 1D and/or 2D NMR spectroscopy. NMR spectroscopy is a powerful technique used to study weak interactions of small ligand-macromol. (-rnM) and information on binding site localization and on dynamic parameters (relaxation times, diffusion coeffs.) at at. level. Using changes in NMR parameters, the stoichiometry of the complex, assocn. or dissocn. const. values, and enthalpic parameters can be calcd. All this information can be obtained for complexes in their natural environment without degrdn. or probe addn. Depending on the type of NMR method used, macromol. or ligand resonances are obsd.