Whole-organ decellularization is recently gaining interest in the transplantation field as strategy to obtain acellular scaffold only composed by extracellular matrix. These structures, that still remain organ-specific in terms of biological cues and tridimensional morphology could be then recellularized with patient's autologous cells. The final result should be a nwe transplantable autologous organ that should by-pass, at the same time, the problem of organ shortage and secondly the consequences related to the immunosuppression need. Herein we describe the protocol to manufacture a whole-organ transplantable rat kidney scaffold by a dual-detergent (Triton X-100 and SDS) arterial peristaltic perfusion. Final results show whole-renal acellular scaffold with contextual preservation of tridimensional architecture and biological properties deriving from the extracellular matrix composition.
CITATION STYLE
Peloso, A., Citro, A., Corradetti, V., Brambilla, S., Oldani, G., Calabrese, F., … Cobianchi, L. (2018). In-lab manufacturing of decellularized rat renal scaffold for kidney bioengineering. In Methods in Molecular Biology (Vol. 1577, pp. 103–110). Humana Press Inc. https://doi.org/10.1007/7651_2017_96
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