Background: Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required. Findings. A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green. Conclusions: The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity. © 2012 Jin-Long et al; licensee BioMed Central Ltd.
CITATION STYLE
Jin-Long, Y., Rui, Y., Ke-Fei, S., Xiang-Wei, P., Tao, X., & Zuo-Hua, L. (2012). Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay. Virology Journal, 9. https://doi.org/10.1186/1743-422X-9-47
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