In vitro antioxidative potential of Artemisia indica Willd

Abstract

There is enough evidence, including epidemiological studies indicating the relationship between the plant antioxidants and reduction of chronic diseases in animals and humans. This paper is the first report regarding the evaluation of in vitro antioxidative potential of Artemisia indica Willd. extracts (AEs), a perennial herb found in western Himalayas and many other parts of world, traditionally utilized as medicine and food for livestock and humans. The dried and powdered aerial parts were Soxhlet extracted with methanol (AME), ethanol (AEE) and 50% hydro-methanol (AHME), to compare the extraction efficacies of these solvents. These respective crude extracts were then subjected to DPPH, H2O2, NO scavenging and total antioxidant capacity (TAC) assays. For scavenging assays, various concentrations of each extract in triplicate, were tested with Quercetin as the standard. All AEs exhibited a significant DPPH, H2O2 and NO scavenging activity in a concentration-dependent manner. Lower to higher order of the IC50value of all three scavenging assays were AME