UTILIZAÇÃO DE RESÍDUOS DA AGROINDÚSTRIA PARA A PRODUÇÃO DE ENZIMAS LIPOLÍTICAS POR FERMENTAÇÃO SUBMERSA

Abstract

Lipases are enzymes capable of cleaving the ester bound of triacylglycerides, and under favorable conditions they can perform synthesis reactions such as esterification and tran- esterification. The microbial lipases are the most used ones because they have some interesting features for their use under organic media. This work aims to optimize the lipase production from the anendophytic strain of Candida guilliermondi by experimental design. The PlackettBurman design was applied with 6 variables of medium composition and inoculum. The variables glycerol and ammonium sulfate showed the greatest effect over the enzyme secretion, 7,2775 ±1,58 and 9,9225±1,58 U.mL-1, respectively. The experiment with all variables at highest level had the greatest lipase secretion, 27,42 U.mL-1, confirming the results obtained at the variable statistical analysis. With the obtained results it is possible to conclude that this endophytic yeast strain is a promising microorganism for lipase to occur and the experimental design is a valuable tool to optimize the metabolite production, like enzymes.