A high-performance liquid chromatography (HPLC) method for the quantitation of doxycycline in bulk, tablets, and capsules after storage at -20, 5, 25, 40, 50, 60, and 70°C, has been developed and validated. The samples are eluted from a μ -Bondapak C8 column (4.6 x 150-mm, i.d., 5-μm particle size) at 27°C, with a mobile phase of acetonitrile-water-THF (29.5:70:0.5, v/v/v), adjusted to pH 2.5 with 1.0M HCl. The flow rate is 1.0 mL/min and detection by UV is at 350 nm. The stability of doxycycline in bulk and in pharmaceuticals is checked over 90 days. Doxycycline shows thermo-degradation after exposure to high temperature; tablets are more stable than capsules. The shelf lives (t 90%) are determined to be 1.00, 2.84, and 5.26 years in bulk, capsules, and tablets, respectively, at 25°C. Metacycline and 6-epidoxycyline are identified as degradation products at high temperatures. Amounts of doxycycline, metacycline, and 6-epidoxycycline in all samples are determined by HPLC, and the results compare with those from micellar electrokinetic capillary chromatography. After 90 days, metacycline and 6-epidoxycyline are almost equal in test samples from standard bulk form, tablets, and capsules. It is 27.8 ± 0.3%, 13.7 ± 0.1%, and 18.8 ± 0.2%, respectively.
CITATION STYLE
Injac, R., Djordjevic-Milic, V., & Srdjenovic, B. (2007). Thermostability testing and degradation profiles of doxycycline in bulk, tablets, and capsules by HPLC. Journal of Chromatographic Science, 45(9), 623–628. https://doi.org/10.1093/chromsci/45.9.623
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