Effect of interferon‐gamma priming on the activation of murine peritoneal macrophages to tumouricidal state by cisplatin, IL‐1, and tumour necrosis factor (TNF): production of IL‐1 and TNF

Abstract

The effect of interferon‐gamma (IFN‐γ) priming of murine peritoneal macrophages on the activation to tumouricidal state by cisplatin. lipopolysaccharide (LPS)‐IL‐1 and TNF was investigated. Cisplatin‐, LPS‐, IL‐1‐ or TNF‐treated IFN‐γ‐primed macrophages showed significantly enhanced tumouricidal activity and binding to tumour cells, compared with unprimed treated or untreated macrophages. Macrophages treated with cisplatin, LPS, IL‐1 and TNF produced released and membrane‐associated IL‐1 and TNF activity which was significantly enhanced after priming with IFN‐γ. These observations suggest the use of IFN‐γ along with these biological response modifiers in designing immunotherapeutic protocols for treatment of malignancy. Copyright © 1992, Wiley Blackwell. All rights reserved