Protein engineering - Methods and protocols

Abstract

Protein engineering : past, present, and future / Stefan Lutz and Samantha M. Iamurri -- Rational and semirational protein design / Ivan V. Korendovych -- Computational analysis of protein tunnels and channels / Jan Brezovsky, Barbora Kozlikova, and Jiri Damborsky -- YASARA : a tool to obtain structural guidance in biocatalytic investigations / Henrik Land and Maria Svedendahl Humble -- Computational library design protocol for rapid improvement of protein stability : FRESCO / Hein J. Wijma, Maximilian J.L.J. Furst, and Dick B. Janssen -- Directed evolution of proteins based on mutational scanning / Carlos G. Acevedo-Rocha, Matteo Ferla, and Manfred T. Reetz -- Brief guide to the high-throughput expression of directed evolution libraries / Ana Luısa Ribeiro, Mario Mencıa, and Aurelio Hidalgo -- Library growth and protein expression : optimal and reproducible microtiter plate expression of recombinant enzymes in E. coli using MTP shakers / Sandy Schmidt, Mark Dorr, and Uwe T. Bornscheuer -- Normalized screening of protein engineering libraries by split-GFP crude cell extract quantification / Javier Santos-Aberturas, Mark Dorr, and Uwe T. Bornscheuer -- Functional analysis of membrane proteins produced by cell-free translation / Srujan Kumar Dondapati, Doreen A. Wustenhagen, and Stefan Kubick -- Practical considerations regarding the choice of the best high-throughput assay / Carolin Mugge and Robert Kourist -- High-throughput screening assays for lipolytic enzymes / Alexander Fulton, Marc R. Hayes, Ulrich Schwaneberg, Jorg Pietruszka, and Karl-Erich Jaeger -- Continuous high-throughput colorimetric assays for α-transaminases / Egon Heuson, Jean-Louis Petit, Franck Charmantray, Veronique de Berardinis, and Thierry Gefflaut -- Colorimetric high-throughput screening assays for the directed evolution of fungal laccases / Isabel Pardo and Susana Camarero -- Directed coevolution of two cellulosic enzymes in Escherichia coli based on their synergistic reactions / Min Liu, Lidan Ye, and Hongwei Yu -- Program-guided design of high-throughput enzyme screening experiments and automated data analysis/evaluation / Mark Dorr and Uwe T. Bornscheuer -- Solid-phase agar plate assay for screening amine transaminases / Martin S. Weiss, Uwe T. Bornscheuer, and Matthias Hohne -- Ultrahigh-throughput screening of single-cell lysates for directed evolution and functional metagenomics / Fabrice Gielen, Pierre-Yves Colin, Philip Mair, and Florian Hollfelder -- Isolation of pH-sensitive antibody fragments by fluorescence-activated cell sorting and yeast surface display / Christian Schroter, Simon Krah, Jan Beck, Doreen Konning, Julius Grzeschik, Bernhard Valldorf, Stefan Zielonka, and Harald Kolmar -- Library generation and auxotrophic selection assays in Escherichia coli and Thermus thermophilus / Jorg Claren, Thomas Schwab, and Reinhard Sterner.