Native electrospray ionization mass spectrometry (native ESI-MS) is a powerful tool to investigate non-covalent biomolecular interactions. It has been widely used to study protein complexes, but only few examples are described for the analysis of complexes involving RNA-RNA interactions. Here, we provide a detailed protocol for native ESI-MS analysis of RNA complexes. As an example, we present the analysis of the HIV-1 genomic RNA dimerization initiation site (DIS) extended duplex dimer bound to the aminoglycoside antibiotic lividomycin.
CITATION STYLE
Wolff, P., & Ennifar, E. (2020). Native Electrospray Ionization Mass Spectrometry of RNA-Ligand Complexes. In Methods in Molecular Biology (Vol. 2113, pp. 111–118). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0278-2_9
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