Magnetic resonance microscopy of chemically fixed human embryos at high spatial resolution

Abstract

We acquired magnetic resonance (MR) microscopic images of chemically fixed human embryos of Carnegie stages 16 to 22 with a large image matrix (256 © 256 © 512) using an MR microscope that we developed with a 9.4-tesla vertical wide-bore superconducting magnet and a dual-channel receiver system to extend the dynamic range of the MR signal. The images showed clear anatomical structures at spatial resolutions of (40 µm) 3 to (60 µm) 3. We concluded that the experimental technique we developed will aid construction of the next anatomical database of the collection of chemically fixed human embryos.