Regulation of Human COL9A1 Gene Expression

  • Zhang P
  • Jimenez S
  • Stokes D
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Abstract

The COL9A1 gene contains two promoter regions, one driving expression of a long α1(IX) chain in cartilage (upstream) and one driving expression of a shorter chain in the cornea and vitreous (downstream). To determine how the chondrocyte-specific expression of the COL9A1 gene is regulated, we have begun to characterize the upstream chondrocyte-specific promoter region of the human COL9A1 gene. Transient-transfection analyses performed in rat chondrosarcoma (RCS) cells, human chondrosarcoma (HTB) cells, and NIH/3T3 cells showed that the COL9A1 promoter was active in RCS cells but not HTB or NIH/3T3 cells. Inclusion of the first intron had no effect on promoter activity. In transient-transfection analyses with promoter deletion constructs, it was found that full promoter activity in RCS cells depended on the region from −560 bp to +130 bp relative to the transcriptional start site (+1). Sequence analysis of the region from −890 bp to the transcriptional start predicted five putative SOX/Sry-binding sites. Mutation analysis revealed that two of three putative SOX/Sry binding sites within the −560 to +130 bp region are responsible for most of the COL9A1 promoter activity in RCS cells. Co-transfection experiments with a SOX9 expression plasmid revealed that a construct containing the five putative SOX/Sry-binding sites was transactivated 20- to 30-fold in both HTB and NIH/3T3 cells. Further co-transfection experiments showed that two of the SOX/Sry-binding sites located within the −560 to +130 bp region were required for full transactivation. However, mutation and deletion analyses indicated that a region from −560 to −357 bp, which does not contain any other conspicuous SOX9 sites, is also important for full promoter activity. DNA-protein binding assays and super-shift analysis revealed that SOX9 can form a specific complex with one of the SOX/Sry-binding sites with in the −560 to +130 region.

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APA

Zhang, P., Jimenez, S. A., & Stokes, D. G. (2003). Regulation of Human COL9A1 Gene Expression. Journal of Biological Chemistry, 278(1), 117–123. https://doi.org/10.1074/jbc.m208049200

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