Improvement of bartonella henselae DNA detection in cat blood samples by combining molecular and culture methods

31Citations
Citations of this article
47Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Bartonella spp. are bacteria of worldwide distribution that cause asymptomatic to fatal infections in animals and humans. The most common zoonotic species is Bartonella henselae, for which cats are the major natural reservoir host. To better understand Bartonella sp. diagnostic limitations, we determined the frequency of bloodstream infection in 112 cats by comparing and combining the results of multiple conventional and nested PCRs from blood and liquid culture samples. Using liquid culture conventional PCR, Bartonella sp. DNA was amplified from 27.7% of samples (31/112) compared to 90.2% of samples (101/112) by combining nested PCR from blood and liquid culture, indicating that PCR testing of more than one type of sample provides better sensitivity than a standalone PCR and that bloodstream infection is very frequent among cats in southeastern Brazil. This study reinforces the need for multistep testing for Bartonella sp. infection to prevent false-negative diagnostic results, even in reservoir hosts such as cats that typically maintain higher bacteremia levels.

Author supplied keywords

Cite

CITATION STYLE

APA

Drummond, M. R., Lania, B. G., De Paiva Diniz, P. P. V., Gilioli, R., Demolin, D. M. R., Scorpio, D. G., … Velho, P. E. N. F. (2018). Improvement of bartonella henselae DNA detection in cat blood samples by combining molecular and culture methods. Journal of Clinical Microbiology, 56(5). https://doi.org/10.1128/JCM.01732-17

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free