Fungus-mediated synthesis of silver nanoparticles (agnp) and inhibitory effect on aspergillus spp. in combination with antifungal agent

Abstract

In this study, A. niger synthesized silver nanoparticules (AgNP) were characterised by using UV-Vis Sphecthrofotometry, Atomic Force Microscopy (AFM) and Transmission Electron Microscope (TEM) Analysis. The antifungal effect of synthesised AgNP and antifungal agent Amphothericin B (Amp-B) combination were investigated against Aspergillus spp. Antimicrobial efficiency were evaluated by Kirby Bauer Agar Disk Diffusion Test. In the end of this study, the particule size of AgNP which biosynthesised on A. niger were measured between 13.2-646.8 nm by AFM. The TEM analysis of AgNPs synthesized on A. niger were determined as a spherical in shape with different sizes 25.5-543.3 nm in the examined regions. The development of antifungal inhibition zone on A. niger and A. flavus was respectively carried out to evaluate on application of A. niger-AgNP; between 0-0.67 mm, 0-0.42 mm, Amp-B; 0.70-1.50 mm, 0- 0.65 mm, A. niger-AgNP+Amp-B; 1.14-2.00 mm, 0-1 mm. According to this study data, antifungal effect of were respectively determined %0.4, %1.4, %2.4; %0.1, %0.45, %0.65 on A. niger and A. flavus. The antifungal inhibition zone occurrence indicated depent of both fungi results, the Amp-B were increase %43.91 of A. niger-AgNP; A. niger-AgNP+Amp-B % 40.84 of Amp-B, A. niger-AgNP+Amp-B% 84.75 of A. niger-AgNP. The statistical evaluation of this study showed that multiple comparison of three application on A. niger and A. flavus were significant (p˂0,005).