Enzymatic bioautography enables the detection of enzyme inhibitors absorbed on a thin-layer chromatography plate. Therefore, it is an assay format that is particularly useful for the detection of inhibitors present in complex mixtures. The inhibition properties of compounds separated by thin-layer chromatography can be directly analyzed to produce an inhibition profile. Here, we describe the conditions to detect inhibitor of the enzymes xanthine oxidase and β-glucosidase immobilized on agar gel.
CITATION STYLE
Ramallo, I. A., Salazar, M. O., & Furlan, R. L. E. (2020). Enzymatic bioautographic methods. In Methods in Molecular Biology (Vol. 2089, pp. 179–189). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0163-1_12
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