Occurrence of cello-oligosaccharides in the apoplast of auxin-treated pea stems

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Abstract

Treatment of pea (Pisum sativum L.) hypocotyl segments with indole-3-butyric acid, which promotes segment elongation, increased the solubilization of both xyloglucan and cellooligosaccharides in the apoplast of auxin-treated pea stems. The cello-oligosaccharides were isolated from the apoplastic solution with a charcoal/Celite column and were identified as cellobiose, cellotriose, and cellotetraose after subsequent thin-layer chromatography and paper electrophoresis. Cello-oligosaccharides in the apoplastic fraction were monitored using cellobiose dehydrogenase. Both xyloglucan and cello-oligosaccharides appeared to be formed concurrently within 30 min after treatment with the auxin, and the cello-oligosaccharides increased with stem elongation even after 2 h. The total activity of cellulase did not increase for up to 4 h.

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Tominaga, R., Samejima, M., Sakai, F., & Hayashi, T. (1999). Occurrence of cello-oligosaccharides in the apoplast of auxin-treated pea stems. Plant Physiology, 119(1), 249–254. https://doi.org/10.1104/pp.119.1.249

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