Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion

15Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.

Abstract

Human immediate early response 2 (IER2) has been characterized as a putative nuclear protein that functions as a transcription factor or transcriptional co-activator in the regulation of cellular responses, and may be involved in the regulation of tumor progression and metastasis. Data from our previous gene expression profile of the human microvascular endothelial cells during capillary morphogenesis showed a significant alteration of IER2 expression, suggesting that IER2 may participate in the regulation of the endothelial cell morphogenesis and angiogenesis. The aim of the present study was to investigate the role of IER2 in cell motility, cell-matrix adhesion and in vitro capillary-like structures formation of the human umbilical vein endothelium cells (HUVECs). IER2 was constitutively expressed in HUVECs, and lentiviral-mediated depletion of IER2 significantly reduced the cell motility, cell-matrix adhesion and capillary-like structures formation of HUVECs. Results also showed that depletion and overexpression of IER2 altered the actin cytoskeleton rearrangement in HUVECs. Furthermore, results from western blot analysis showed that the activity of the focal adhesion kinase (FAK) can be regulated by IER2. These results indicated that IER2 regulates endothelial cell motility, adhesion on collagen type I matrix and the capillary tube formation, as the result of the regulation of the actin cytoskeleton rearrangement presumably via a FAK-dependent mechanism.

References Powered by Scopus

Angiogenesis in health and disease

3684Citations
N/AReaders
Get full text

Role of angiogenesis in tumor growth and metastasis.

2602Citations
N/AReaders
Get full text

Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures.

1056Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Transcriptome meta-analysis reveals differences of immune profile between eutopic endometrium from stage I-II and III-IV endometriosis independently of hormonal milieu

64Citations
N/AReaders
Get full text

Urinary single-cell sequencing captures kidney injury and repair processes in human acute kidney injury

26Citations
N/AReaders
Get full text

IER2-induced senescence drives melanoma invasion through osteopontin

19Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Wu, W., Zhang, X., Lv, H., Liao, Y., Zhang, W., Cheng, H., … Shen, W. (2015). Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion. International Journal of Molecular Medicine, 36(4), 1104–1110. https://doi.org/10.3892/ijmm.2015.2310

Readers over time

‘16‘17‘18‘19‘20‘21‘22‘23‘24‘2502468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 6

60%

Researcher 3

30%

Professor / Associate Prof. 1

10%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 10

67%

Medicine and Dentistry 3

20%

Sports and Recreations 1

7%

Agricultural and Biological Sciences 1

7%

Article Metrics

Tooltip
Mentions
References: 1

Save time finding and organizing research with Mendeley

Sign up for free
0