Here, we describe a procedure of human iPS cells differentiation into the definitive endoderm, further into albumin-expressing and albumin-secreting hepatocyte, using M15, a mesonephros- derived cell line. Approximately 90 % of human iPS cells differentiated into SOX17-positive definitive endoderm then approximately 50 % of cells became albumin-positive cells, and secreted ALB protein. This M15 feeder system for endoderm and hepatic differentiation is a simple and efficient method, and useful for elucidating molecular mechanisms for hepatic fate decision, and could represent an attractive approach for a surrogate cell source for pharmaceutical studies.
CITATION STYLE
Umeda, K., Shiraki, N., & Kume, S. (2016). Hepatic differentiation from human ips cells using M15 Cells. In Methods in Molecular Biology (Vol. 1357, pp. 375–381). Humana Press Inc. https://doi.org/10.1007/7651_2014_146
Mendeley helps you to discover research relevant for your work.