Lysobacter agri sp. nov., a bacterium isolated from soil

Abstract

A bacterial strain, designated as THG-SKA3 T, was isolated from field soil of Kyung Hee University, South Korea. Cells of the isolate were observed to be Gram-negative, aerobic, rod-shaped and motile by gliding. The strain was found to grow optimally at 28 °C, at pH 7 and in absence of NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-SKA3 T shared highest sequence similarity with Lysobacter niastensis KACC 11588 T followed by Lysobacter panacisoli KACC 17502 T, Lysobacter enzymogenes LMG 8762 T and Lysobacter oryzae KCTC 22249 T. The G+C content of THG-SKA3 T was determined to be 68.9 mol%. The DNA–DNA relatedness values between strain THG-SKA3 T and its closest phylogenetic neighbors were below 25.0 %.The major polar lipids of strain THG-SKA3 T were determined to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was identified as ubiquinone 8 (Q-8). The major cellular fatty acids were identified as branched chain iso-C 15:0, iso-C 16:0 and unsaturated iso-C 17:1 ω9c. On the basis of polyphasic data presented, it is evident that strain THG-SKA3 T represents a novel species of the genus Lysobacter, for which the name Lysobacter agri sp. nov. (type strain THG-SKA3 T = KACC 18283 T = CSCTCC AB 2015126 T) is proposed.