Evaluation Antibacterial Activity of Quaternary-Based Chitin/Chitosan Derivatives In Vitro

Abstract

Total of 3 water-soluble quaternary-based chitin/chitosan derivatives, which have an identical molecular weight and anion, were synthesized and characterized. Their antibacterial activities against Salmonella cholerae-suis and Bacillus subtilis were evaluated in vitro. The polysaccharides exhibited the antibacterial efficiency. Their minimum inhibitory concentration (MIC) values vary from 0.02 to 20.48 mg/mL, and their minimum bactericidal concentration (MBC) values vary from 0.08 to 40.96 mg/mL against S. cholerae-suis and B. subtilis, respectively. Futhermore, the extent of Bacillus subtilis cells damage was examined via transmission electron microscopy (TEM) to show how N,N,N-trimethylchitosan (TMC) gradually destroyed and killed B. subtilis cells when they were treated with TMC. One of those quaternary polymers, O-([2-hydroxy-3-trimethylammonium])propyl chitin (OHT-chitin), which can be directly and easily synthesized from chitin in bulk quantities, also was demonstrated its antibacterial activity. These water soluble quaternary-based chitin/chitosan derivatives that have antibacterial effect should be potentially used as antimicrobial agents in many fields. Practical Application: The main practical application behind the investigation and evaluation antibacterial activity of 3 water-soluble quaternary-based chitin/chitosan derivatives could be potentially used as antimicrobial agents in many fields. These polysaccharides represent a renewable source of natural biodegradable polymers and meet with the emergence of more and more food safe problems. © 2012 Institute of Food Technologists®.