Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracel-lular survival of GBS.
CITATION STYLE
Lione, V. de O. F., dos Santos, M. H. B., de Oliveira, J. S. S., Mattos-Guaraldi, A. L., & Nagao, P. E. (2014). Interferon-γ inhibits group B streptococcus survival within human endothelial cells. Memorias Do Instituto Oswaldo Cruz, 109(7), 940–943. https://doi.org/10.1590/0074-0276140201
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