Molecular Tension Microscopy has been increasingly used in the last years to investigate mechanical forces acting in cells at the molecular scale. Here, we describe a protocol to image the tension of the junctional protein E-cadherin in cultured epithelial cells undergoing Epithelial-Mesenchymal Transition (EMT). We report how to prepare cells and induce EMT, and how to acquire, analyze, and quantitatively interpret FRET data.
CITATION STYLE
Canever, H., Carollo, P. S., Fleurisson, R., Girard, P. P., & Borghi, N. (2020). Molecular tension microscopy of E-cadherin during epithelial-mesenchymal transition. In Methods in Molecular Biology (Vol. 2179, pp. 289–299). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0779-4_22
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