Human α1-microglobulin levels in various body fluids

Abstract

α1-Microglobulin levels in serum and urine were estimated by using single radial immunodiffusion, resulting in the following mean values: umbilical cord blood serum, 40.6 mg/l; normal adult serum, 44.2 mg/l; and normal urine, 5.7 mg/24 hr urine volume. Slightly higher levels of serum α1-microglobulin were found in infants and the aged. Serum and urine α1-microglobulin levels in patients with renal failure, however, were greatly increased, mean levels being 231.5 mg/l and 100.7 mg/24 hr urine volume, respectively. Serum α1-microglobulin levels in these patients correlated well with both serum creatinine and β2-microglobulin levels. Serum α1-microglobulin level did not correlate positively with serum levels of other plasma proteins, such as α1-antitrypsin, haptoglobin, complement, etc. Ouchterlony immunodiffusion also revealed the presence of α1-microglobulin in synovial fluid, ascites, pleural effusion, amniotic fluid, cyst fluid, and cerebrospinal fluid. The levels of α1-microglobulin in these fluids were measured by single radial immunodiffusion, except that its level in cerebrospinal fluid was measured by radioimmunoassay. Mean α1-microglobulin concentration was 20.8 mg/l in synovial fluid, 28.7 mg/l in ascites, 21.5 mg/l in pleural effusion, 2.7 mg/l in amniotic fluid, 8.2 mg/l in cyst fluid, and 42.3 ng/ml in cerebrospinal fluid.