The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.
CITATION STYLE
Çelebier, M., Ibáñez, C., Simó, C., & Cifuentes, A. (2019). A foodomics approach: Ce-ms for comparative metabolomics of colon cancer cells treated with dietary polyphenols. In Methods in Molecular Biology (Vol. 1855, pp. 303–313). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8793-1_26
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