Antioxidant properties of gomphrena globosa leaves Extract

Abstract

Free radical in the body has known to caused oxidative stress which implicated in pathophysiology. Free radical accumulation can be overcome by the abundance of antioxidants that can inhibit the oxidation reaction. The purpose of this study was to identify the bioactive compound and antioxidant activity of Gomphrena globosa leaf extract (GGLE) on kidney cells (Vero cells) as an excretory organ. Extraction was prepared by maceration using 96% ethanol for 24h. TLC analysis was performed to determine the phytochemical content of GGLE. The antioxidant effect of GGLE was examined by free radical scavenging method using DPPH as the free radical and NO production assay using Griess reagent was also examined in the Vero cell. Cytotoxicity of GGLE on Vero cell was observed with WST-1 assay. The result showed that GGLE contains flavonoid, saponin and terpene compound. The IC50 value of antioxidant activities of GGLE was 100 ± 6.095 µg/mL. The WST-1 assay showed that GGLE was non-toxic for the Vero cells and may inhibit NO production of LPS-induced Vero cell. These inventions exhibit a correlation between the phytochemical content of G.globosa leaves and the value of reducing free radical which indicates ethanol extract of G. globosa leaves has antioxidant properties and may serve as natural antioxidants to prevent cellular damage induced by oxidative stress.