Multilateral budding in the apiculate yeast genera Hanseniaspora, Nadsonia, Saccharomycodes, Wickerhamia, and Kloeckera

Abstract

In addition to the usual bipolar budding, the multilateral budding that developed at different lateral sites on a single cell was observed in 25 strains belonging to the apiculate yeast genera Hanseniaspora, Nadsonia, Saccharomycodes, Wickerhamia, and Kloeckera, Effects of several culture conditions on the formation of this multilateral budding were also examined. The rates of this multilateral budding were about 0.001 to 4.0%, but the rates varied with kinds of media and yeast species, and several yeasts showed multilateral budding at pH 6.0-6.5. Conidiogenesis on lateral sites, as well as bipolar budding, was of the percurrent type by basipetal succession. In addition to lemon-shaped cells, cells of various shapes resulted from this multilateral budding. Thus this multilateral budding can be supplemented as one of the diagnostic characters of the above apiculate yeast genera. © 1982, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation. All rights reserved.