Proteolytic cascade enzymes increase in focal cerebral ischemia in rat

369Citations
Citations of this article
43Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Cerebral infarction initiates a cascade of molecular events, leading to proteolytic cell death. Matrix-degrading metalloproteinases (MMPs) are neutral proteases involved in extracellular matrix damage. Type IV collagenase is an MMP that increases cerebral capillary permeability after intracerebral injection and may be important along with plasminogen activators (PA) in secondary brain edema in stroke. Therefore, we measured MMPs and PAs in spontaneously hypertensive (SHR) or Wistar-Kyoto (WKY) rats with permanent middle cerebral artery occlusion (MCAO). Brain tissue was assayed for MMPs and PAs at 1, 3, 12, and 24 h and 5 days after occlusion, using substrate gel polyacrylamide electrophoresis (zymography). SHR showed an increase in 92-kDa type IV collagenase (gelatinase B) in the infarcted hemisphere compared with the opposite side at 12 and 24 h (p< 0.05). Gelatinase A remained the same in both infarcted and normal tissue until 5 days after injury, when it increased significantly (p < 0.05). Urokinase- type PA was increased significantly at 12 and 24 h and 5 days, while tissue- type PA was decreased significantly at 1, 12, and 24 h in the ischemic compared with the nonischemic hemisphere. Gelatinase B was markedly increased in SHR at 12 and 24 h compared with WKY (p < 0.05). Secondary vasogenic edema is maximal 1-2 days after a stroke, which is the time that gelatinase B was elevated. The time of appearance of gelatinase B suggests a role in secondary tissue damage and vasogenic edema, while gelatinase A may be involved in tissue repair.

References Powered by Scopus

Calcium-mediated neurotoxicity: relationship to specific channel types and role in ischemic damage

1660Citations
N/AReaders
Get full text

Metastatic potential correlates with enzymatic degradation of basement membrane collagen

1618Citations
N/AReaders
Get full text

Calcium fluxes, calcium antagonists, and calcium-related pathology in brain ischemia, hypoglycemia, and spreading depression: A unifying hypothesis

1116Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Metalloproteinases in biology and pathology of the nervous system

930Citations
N/AReaders
Get full text

Effects of matrix metalloproteinase-9 gene knock-out on the proteolysis of blood-brain barrier and white matter components after cerebral ischemia

921Citations
N/AReaders
Get full text

Matrix metalloproteinase-mediated disruption of tight junction proteins in cerebral vessels is reversed by synthetic matrix metalloproteinase inhibitor in focal ischemia in rat

907Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Rosenberg, G. A., Navratil, M., Barone, F., & Feuerstein, G. (1996). Proteolytic cascade enzymes increase in focal cerebral ischemia in rat. Journal of Cerebral Blood Flow and Metabolism, 16(3), 360–366. https://doi.org/10.1097/00004647-199605000-00002

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 18

60%

Professor / Associate Prof. 6

20%

Researcher 6

20%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 8

29%

Medicine and Dentistry 8

29%

Neuroscience 7

25%

Pharmacology, Toxicology and Pharmaceut... 5

18%

Save time finding and organizing research with Mendeley

Sign up for free