An Analysis of In Vitro Antioxidant and Anti-inflammatory Activities of Mucuna pruriens (Leaves) and Allium sativum (Bulbs)

Abstract

The anti-inflammatory steroidal and non-steroidal drugs are commonly used to treat inflammation. Be that as it may, with a number of side effects, these are disabled. We have therefore selected two plants, i.e., Mucuna pruriens and Allium sativum, which have various therapeutic agents used in traditional medicines and Ayurveda. The objective of this investigation was to test in vitro anti-inflammatory activities of Mucuna pruriens leaves and Allium sativum bulb extracts of both either alone or in combination by evaluating inhibition of cyclooxygenase and with determination of protein denaturation. The successive solvent extraction of shade dried leaves and peeled dry bulbs was performed for both plants with solvents of increased polarity. Then the two plants’ in vitro antioxidant assay was evaluated by estimating DPPH, H2O2, NO, ABTS and ascorbic acid as standard at various concentrations. The methanol and combined methanol extracts from the two plants showed significant antioxidant activity on DPPH, H2O2, NO and ABTS among the four extracts. In addition, the cyclooxygenase inhibition (76.23%) and protein denaturation (65%) were also tested for anti-inflammatory activity, which revealed that these two plants had strong antioxidant and anti-inflammatory principles.