Asessing Tissue-Specific Autophagy Flux in Adult Caenorhabditis elegans

4Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The cellular recycling process of autophagy is essential for survival, development, and homeostasis. Autophagy also plays an important role in aging and has been linked to longevity in many species, including the nematode C. elegans. Study of the physiological roles of autophagy during C. elegans aging requires methods for the spatiotemporal analysis of autophagy. Here we describe a method for assessing autophagic flux in multiple tissues of C. elegans by quantifying the pool of autophagic vesicles using fluorescently labelled Atg8/LGG-1 reporters upon autophagy inhibition using bafilomycin A1 (BafA). This methodology has revealed that autophagic activity varies in different cell types of C. elegans during aging.

Cite

CITATION STYLE

APA

Chang, J. T., Hansen, M., & Kumsta, C. (2020). Asessing Tissue-Specific Autophagy Flux in Adult Caenorhabditis elegans. In Methods in Molecular Biology (Vol. 2144, pp. 187–200). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0592-9_17

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free