Transcriptome analysis of membrane transporters in response to salinity stress

Abstract

Exposure to high ambient levels of NaCl affects plant water relations and creates ionic stress. To a large extent, responses to such stress depend on the action of membrane transporters, particularly those that move cations such as Na+ and K+. A genomics approach can greatly help with the identification of important membrane transporter genes. This can be done by comparing transcriptomes of salinized and non-salinized plants, by comparing tolerant and non-tolerant species, or by using intraspecies variation. This chapter describes a protocol using oligo-microarrays to compare salinity treated (50 mM NaCl) and non-treated rice roots, presenting protocols for growth, RNA isolation, cDNA synthesis and labeling, and a summary of data collection, analysis, and interpretation. Although focused on rice root tissue, the described procedures can be applied to many different treatments, tissues, and plant species. © 2012 Springer Science+Business Media, LLC.