Bio-ethanol production from sweet sorghum bagasse by engineered simultaneous saccharification and fermentation technology using Trichoderma reesei and Saccharomyces cerevisiae

Abstract

This study was performed on sweet sorghum bagasse as a medium for bio-ethanol production. The aim of this study is to design a method to produce bio-ethanol by Simultaneous Saccharification and Fermentation (SSF) technology using Trichoderma reesei and Saccharomyces cerevisiae at a higher yield. Pre-treatment stage was conducted by using sodium hydroxide (2%) hydrothermal and hydrogen peroxide (7.44%) for 6 h, at temperature of 40°C and agitation of 120 rpm. The experimental setup consisted of two treatments, namely Conventional SSF (CSSF) and Engineered SSF (ESSF). The results show that Conventional SSF using full agitation and aeration can produce bio-ethanol at 6.6 g L-1, with the yield (Y p/s) of 0.17 g bio-ethanol/g substrate, and productivity of 0.09 g L-1 h-1. A better result is obtained using Engineered SSF which produces bio-ethanol at 10.73 g L-1, with the yield (Y p/s) of 0.33 g bio-ethanol/g substrate and productivity of 0.15 g L-1 h-1.