Doubled haploid (DH) techniques using immature microspores or embryos rescued in vitro following interspecific hybridization are important tools for breeding wheat and barley and for generating populations for gene mapping purposes. Here, we describe methods being used for the successful production of spring wheat and barley DHs in Australia.
CITATION STYLE
Broughton, S., Sidhu, P. K., & Davies, P. A. (2014). In vitro culture for doubled haploids: Tools for molecular breeding. Methods in Molecular Biology, 1145, 167–189. https://doi.org/10.1007/978-1-4939-0446-4_14
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