Recombinant human butyrylcholinesterase as a new-age bioscavenger drug: Development of the expression system

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Abstract

Butyrylcholinesterase (BChE) is a serine hydrolase (EC 3.1.1.8) which can be found in most animal tissues. This enzyme has a broad spectrum of efficacy against organophosphorus compounds, which makes it a prime candidate for the role of stoichiometric bioscavenger. Development of a new-age DNA-encoded bioscavenger is a vival task. Several transgenic expression systems of human BChE were developed over the past 20 years; however, none of them has been shown to make economic sense or has been approved for administration to humans. In this study, a CHO-based expression system was redesigned, resulting in a significant increase in the production level of functional recombinant human butyrylcholinesterase as compared to the hitherto existing systems. The recombinant enzyme was characterized with Elman and ELISA methods. © 2013 Park-media, Ltd.

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Ilyushin, D. G., Haertley, O. M., Bobik, T. V., Shamborant, O. G., Surina, E. A., Knorre, V. D., … Ponomarenko, N. A. (2013). Recombinant human butyrylcholinesterase as a new-age bioscavenger drug: Development of the expression system. Acta Naturae, 5(16), 73–84. https://doi.org/10.32607/20758251-2013-5-1-73-84

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