Modelling of diseased tissue diffuse reflectance and extraction of optical properties

Abstract

The measurement of optical properties of biological tissues remains a central problem in the field of biomedical optics. Knowledge of these parameters is important in both therapeutic and diagnostic applications of light in medicine. In this work, based on the diffuse reflectance spectra obtained through experimentation the optical properties are determined. Reflectance spectra are obtained by setting up a simple diffuse reflectance spectroscopic system based on fiber optics. Light from a white light source is incident onto the tissue site through the reflectance probe and the backscattered light is collected by the same and impinged on a spectrometer which generates a spectrograph which is acquired in the system. Empirical forward light transport model based on diffusion theory is formulated. The reflectance spectra obtained is fitted to the proposed spectral (analytical) model. The model coefficients determined by non-linear least square optimization method are used to determine the optical properties of the tissue. © 2011 Springer Science+Business Media B.V.