During vertebrate development, Erk is activated and regulates multiple cellular processes such as cell growth, differentiation, migration, and adhesion in a spatiotemporal manner. Whole-mount immunohistochemistry using antibodies against diphosphorylated Erk (p-Erk; active form of Erk) is a very useful method for understanding the spatial and temporal patterns of Erk activity during embryonic development. However, the fixation step of this method stops embryo development at a certain time point, making it very difficult to observe and interpret Erk activity dynamics. In this chapter, we describe a strategy that combines immunohistochemistry and quantitative analyses of multiple fixed embryos to reconstruct Erk activity dynamics during zebrafish somitogenesis.
CITATION STYLE
Matsui, T., & Bessho, Y. (2017). Analyzing ERK signal dynamics during zebrafish somitogenesis. In Methods in Molecular Biology (Vol. 1487, pp. 367–378). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6424-6_27
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