Green synthesis concept of nanoparticles from environmental bacteria and their effects on pathogenic bacteria

Abstract

Soil bacteria play an interesting role in the reduction of Ag+ ions and the formation of silver nanoparticles (AgNPs), which may be a good source for nanoparticles and play a major role in nanotechnology applications. The concept of this project was to study the effects of these environmentally produced nanoparticles on the growth of some pathogenic bacteria. The environmental bacteria were isolated from soil, purified on broth cultures, and centrifuged, while the supernatant was extracted to detect its ability to convert silver nitrate to nanoparticles. The AgNPs was detected by Atomic Force Microscopy (AFM), while Granularity Cumulating Distribution (GCD) was employed to estimate the AgNPs sizes. The results showed the synthesis of AgNPs with sizes of 63.50nm and 45.81nm from the extracts of environmental Pseudomonas sp. and Enterobacter, respectively. The synthesized AgNPs from the extracts of all environmental bacteria showed antibacterial activity against some pathogenic bacteria (Gram positive and Negative) with variable inhibition zones. In conclusion, environmental bacteria can be a cheap source of nanoparticles.