Naturally occurring proteins often consist of multiple distinct domains joined by linker regions. Similarly, the ability to combine globular protein domains through engineered linkers would allow the creation of a wide variety of complex and useful multifunctional proteins. Recent advances in computational design of protein structures have enabled highly accurate design of novel protein structures. In this chapter we outline a computational protocol for the de novo design of protein linkers, and apply this protocol to the design of a helical linker between two rigid protein domains.
CITATION STYLE
Kuhlman, B., Jacobs, T., & Linskey, T. (2016). Computational design of protein linkers. In Methods in Molecular Biology (Vol. 1414, pp. 341–351). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3569-7_20
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