WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells

Abstract

WST-8 is one of the newer generation formazan-based dyes, which release the converted product into the medium in a soluble form. This allows for a non-destructive determination of viability enabling the cells to be subject to further investigations. This is a major advantage in cases where cell phenotype is being investigated and data such as matrix synthesis is correlated to cell number. This chapter describes the use of WST-8 to normalise alkaline phosphatase activity.