Basement membrane type IV collagen molecules in the choroid plexus, pia mater and capillaries in the mouse brain

72Citations
Citations of this article
36Readers
Mendeley users who have this article in their library.

Abstract

We investigated the differential distribution of basement membrane type IV collagen α chains in the mouse brain by immunohistochemistry using α chain-specific monoclonal antibodies. Subendothelial basement membranes were found to contain α1 and α2 chains. Basement membranes surrounding smooth muscle cells on blood vascular walls were immunoreactive for α1 and α2 chains but not for α5 and α6 chains. Interestingly, the pia mater contained a thin basement membrane which was positive for α1, α2, α5, and α6 chains, suggesting that glia limitans superficialis coheres basement membranes containing [α1(IV)]2α2(IV) and [α5(IV)2α6(IV) molecules. In contrast, capillaries always possessed thin basement membranes of [α1(IV)]2α2(IV) molecules. Cerebrospinal fluid is produced through filtration of blood at the choroid plexus, where two distinct basement membranes were detected by anti-α1 and anti-α2 antibodies. The subendothelial basement membrane appeared to consist of [α1(IV)]2α2(IV) molecules, whereas the subependymal basement membrane in the choroid plexus was strongly positive for α3, α4, and α5 chains, indicating that the filtering unit was composed of α3(IV)α4(IV)α5(IV) molecules. That the specific localizations of these molecules are shared by renal glomeruli and the choroid plexus leads us to hypothesize that the supramolecular network containing a3(IV) α4(IV)α5(IV) molecules may function as a permeability selective barrier.

Cite

CITATION STYLE

APA

Urabe, N., Naito, I., Saito, K., Yonezawa, T., Sado, Y., Yoshioka, H., … Ninomiya, Y. (2002). Basement membrane type IV collagen molecules in the choroid plexus, pia mater and capillaries in the mouse brain. Archives of Histology and Cytology, 65(2), 133–143. https://doi.org/10.1679/aohc.65.133

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free