The potential affibodies in new cancer marker immunosensors

Abstract

In this paper, a simple and sensitive immunoassay for a tumor marker human epidermal growth factor receptor 2 (HER2) detection is presented. The immunoassay is based on a sandwich format in which modified magnetic microparticles were coupled to multiplexed electrochemical detection platform. The antibody modified beads are used to capture the protein from the sample solution and the sandwich assay is performed by adding Anti-ErbB2 affibody labeled with biotin. The enzyme alkaline phosphatase (AP) conjugated with streptavidin and its substrate (α-naphthyl-phosphate) were then used for the electrochemical detection by a multiplexed electrochemical platform. The immunosensor developed promise to be a sensitive, multiplexed tool for fast and easy HER2 multianalysis. © 2012 Springer Science+Business Media, LLC.