QCM based on flow system for cardiovascular disease

Abstract

Quartz Crystal Microbalance (QCM) is a special sensor which has acoustic impedance detector by mass loading. In this study Cardiac Troponin T (CTnT) which elevates in all patients with AMI diagnosed by World Heath Organization (WHO) criteria is used as immunoligical assay onto sensor. Detection of the Troponin T is developed based on antigen - antibody system on QCM sensor as solid support and signal transduction for immobilization to monitor risk marker of myocardial infarction. To immobilize antibody, the sensor is functionalized with Polyvinyl chloride (PVC) doped COOH by spray coating technique. The modified sensor testing results can be given in short response time and a direct conversion of mass accumulation into a frequency shift representing a measurable electrical signal. The relationship between the Cardiac Troponin T concentration and the response current could be observed in the minimum concentration range at 5 ng/ml. © 2009 Springer-Verlag.