Background/Aim: OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1) is a long noncoding RNA located on human chromosome 15q15.1 and transcribed in the opposite direction to OIP5. Here, we report that OIP5-AS1 is involved in regulating cell proliferation. Materials and Methods: HeLa cells were transfected with OIP5-AS1-targeting siRNA oligonucleotides and anti-sense oligonucleotides. The cells were harvested 72 h after transfection and subjected to quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and cell-cycle and apoptosis analysis. Results: OIP5-AS1 was expressed at a lower level in cells harbouring an oncogenic kirsten rat sarcoma viral oncogene homolog (K-RAS) mutation than in cells expressing wild-type K-RAS. Silencing OIP5-AS1 with siRNA oligonucleotides or anti-sense oligonucleotides reduced HeLa cell proliferation. Apoptosis and cell-cycle analysis showed that silencing OIP5-AS1 did not cause apoptosis, but did cause G2/M phase cell-cycle arrest. Conclusion: These results suggest that OIP5-AS1 positively regulates cell proliferation by promoting G2/M phase progression.
CITATION STYLE
Naemura, M., Kuroki, M., Tsunoda, T., Arikawa, N., Sawata, Y., Shirasawa, S., & Kotake, Y. (2018). The long noncoding RNA OIP5-AS1 is involved in the regulation of cell proliferation. Anticancer Research, 38(1), 77–81. https://doi.org/10.21873/anticanres.12194
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