Sequencing and characterization of the sdaB gene from Escherichia coli K‐12

Abstract

The sdaB gene which codes for the second l‐serine deaminase (L‐SD) of Escherichia coli K‐12 has been sequenced and shown to be very similar to the sdaA gene which codes for the first l‐serine deaminase. sdaB is transcribed in rich medium, particularly in the absence of glucose, and is under the control of catabolite activator protein. A mutation which established expression of the sdaB gene and synthesis of l‐serine deaminase 2 in minimal medium has been demonstrated to result in a change in the ribosome‐binding site of the sdaB gene. Copyright © 1993, Wiley Blackwell. All rights reserved