Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome. Unbiased whole genome sequencing comparing parents to progeny has revealed that the rate of Cas9-induced mutagenesis in mouse embryos is indistinguishable from the background rate of de novo mutation. However, establishing the best practice to confirm on-target alleles of interest remains a challenge. We believe that improvement in editing strategies and screening methods for founder mice will contribute to the generation of quality-controlled animals, thereby ensuring reproducibility of results in animal studies and advancing the 3Rs (replacement, reduction, and refinement).
CITATION STYLE
Ayabe, S., Nakashima, K., & Yoshiki, A. (2019). Off- and on-target effects of genome editing in mouse embryos. Journal of Reproduction and Development, 65(1), 1–5. https://doi.org/10.1262/jrd.2018-128
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