0271 The change of oxidative DNA damage in nurses with shift work

Abstract

OBJECTIVES: To investigate the change of concentration of 8-hydroxy-2'-deoxyguanosine (8-OHdG) at different work time among shift work nurses.METHOD: A total of 51 nurses with regulated rotation shift in medical centre or district hospital. Urinary concentrations of 8-OHdG, a biomarker of oxidative DNA damage, were measured. According the rotation schedule, like 1-2 days off - 2day shifts-2evenning shifts- 2night shifts, the urine was collected at 4 time points. The 2 workdays on the last day shift and last night shift of a shift course were selected and urine was collected at work before and finished work after on assigned workday, separately.RESULTS: The urinary concentrations of 8-OHdG at work after was significantly higher than at work before on the end of day shift (paired t test, p-value: 0.0363). The concentrations of 8-OHdG between at work after and work before on the end of nigh shift were not significantly different (paired t test, p-value: 0.1673). Besides, there was no difference on the concentrations of 8-OHdG at work after between a day shift and a night shift. We also found that the change of concentrations of 8-OHdG at a day shift was significantly different on work institution (p-value: 0.0009).CONCLUSIONS: We found the more change of concentration of 8-OHdG was at a day shift work then a night shift work, even night shift was as a risk factor of health. Therefore, the change of concentration of 8-OHdG was as a biomarker to respond the workload exposure.