ENZYMATIC REGENERATION OF ATP FROM AMP AND ADP - 2. ENZYME IMMOBILIZATION AND REACTOR DEVELOPMENT.

Abstract

Results of an enzymatic regeneration study are presented which show that the enzymatic regeneration of ATP from AMP and/or ADP using acetyl phosphate, acetate kinase and adenylate kinase is an entirely practical proposition. Acetyl phosphate appears to be readily available, the two enzymes have good stability under the operating conditions, and the thermodynamics of the coupled enzymatic reaction system are attractive for possible use in large scale reactors. Of the various schemes that have been proposed for enzymatic regeneration of ATP, this scheme seems to have the most appeal, because it is capable of handling both ADP and AMP, and because the phosphate source on which it ultimately relies appears to be the most economical.