In retinal diseases characterized by photoreceptor degeneration, the main cause of clinically significant vision loss is cone, rather than rod, loss. Photoreceptor apoptosis can be affected by the availability and/or delivery of neurotrophic proteins, and cultures of photoreceptors are valuable for studying these processes. In the present study, a technique was designed to purify cones to make it possible to screen for neuroprotective molecules. The present chapter describes a technique for preparing cultures of purified rat retina cone photoreceptors based upon panning with peanut agglutinin lectin, which selectively binds to cones. In addition, we describe a protocol for the purification and culture of retinal pigmented epithelial cells from postnatal rat. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Skaper, S. D. (2012). Isolation and culture of rat cone photoreceptor cells. Methods in Molecular Biology, 846, 147–158. https://doi.org/10.1007/978-1-61779-536-7_13
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