A colorimetric and fluorescent pH probe for imaging in E. coli cells

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Abstract

A colorimetric and fluorescent pH probe 1-(4-methylphenyl)-3-(4-dimethylaminophenyl) acrylketone (MDAK) was facilely prepared. The probe exhibited strong pH-dependent behavior and responded linearly and rapidly to minor pH fluctuations within the range of 1.53-3.96. In addition, MDAK displayed a notably large Stokes shift of 121 nm. MDAK possessed a highly selective response to H+ over other metal ions, amion acids and displayed good photostability and excellent reversibility. Furthermore, it can be applied to visualize extreme acidity in E. coli cells.

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Chao, J., Song, K., Wang, H., Li, Z., Zhang, Y., Yin, C., … Zhang, T. (2017). A colorimetric and fluorescent pH probe for imaging in E. coli cells. RSC Advances, 7(2), 964–970. https://doi.org/10.1039/c6ra24885c

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