Molecular cloning of an EcoRI fragment which contains 4.5s and 5s RNA genes in tobacco chloroplasts

Abstract

A 0.9×106 dalton EcoRI fragment of tobacco chloroplast DNA has been cloned in E. coli plasmid pMB9. This fragment contains the chloroplast 4.5S and 5S ribosomal RNA genes. A physical map of the recombinant plasmid was constructed by cleavage with several restriction endonucleases and Southern hybridization with 32P-labeled 4.5S and 5S RNAs. © 1980, The Genetics Society of Japan. All rights reserved.