In this paper, we describe the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The effectiveness and usefulness of SERS-based fiber-optic nanoprobes are illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized "normal" human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor cellular microenvironments at the single cell level. © 2008 Springer-Verlag.
CITATION STYLE
Scaffidi, J. P., Gregas, M. K., Seewaldt, V., & Vo-Dinh, T. (2009). SERS-based plasmonic nanobiosensing in single living cells. Analytical and Bioanalytical Chemistry, 393(4), 1135–1141. https://doi.org/10.1007/s00216-008-2521-y
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