The study of ethanolic extract of binahong leaves (Anredera cordifolia [Ten.] Steenis) and mulberry leaves (Morus nigra L.) in combination on hyperlipidemic-induced rats

Abstract

Objective: This study aimed to determine the antihyperlipidemic activity of binahong leaves and murbey leaves extracts in combination. Methods: A total of 28 rats were divided into seven groups: normal, control, simvastatin 3.6 mg/kg bw, binahong leaves extract 100 mg/kg bw (B100), mulberry leaves extract 200 mg/kg bw (M200), combination of binahong extract 50 mg/kg and mulberry 100 mg/kg (B50+M100), and combination of binahong extract 100 mg/kg bw and mulberry 200 mg/kg bw (B100+M200). Hyperlipidemia was induced by giving rats a high-fat diet along with cholesterol (200 mg/kg), cholic acid (0.2% of chow), and propylthiouracil (10 mg/kg bw) for 30 days orally. Then, for the next 14 days, all groups except the control were given the tested substances. Measurement of lipid profile was performed immediately after induction (T0), 7 and 14 days after treatment (T7 and T14, respectively). Results: There were significant differences on a group of M200, B50+M100, and B100+M200 compared to the control group at T14, with the value 33.68%, 34.39 %, and 44.81% (in reduction of total cholesterol [TC]) and 36.86%, 37.16%, and 49.99%, respectively (in reduction of triglyceride [TG]). Conclusion: Group of M200, B50+M100, and B100+M200 reduced TC and TG level remarkably at day 14. The combination of both extract (B100+M200) considerably shows better activity than either binahong extract or mulberry extract.